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Quantitative Estimation of Photochemical in the Whole Plant Extract of Lycopus Sinnatus (Nutt.) Benth and Their Tests for Allelopathic Effect on Crop seed Germination | Original Article

Parimita Purohit*, Abinash Pradhan, in Anusandhan | Technology & Management


The plant authenticated as Lycopus sinnatus (Nutt.) Benth is a common weed of crop fields collected from near the maize crop field of O.U.A.T. farm campus during September 2015 to October 2015.The aquous extract of the whole plant was prepared by Soxhalation. The quantity of Alkaloids, Flavonoids and Phenols estimated are 980.46mgATE100gmDW, 713.06mgQUE100gmDW and 827.66mgGAE100gmDW respectively. Two different concentrations of water solution of the extract i.e. 0.906 and 1.188 were tested for their effect on seed germination of Helianthus annus L., Lycopersicon esculentum L.,Triticum aestivum L., Oryza sativa L. and Vigna mungo L. The of germination in control is 96 in H.annus,90 in L.esculentum,96 in T.aestivum,80 in O. sativa,28 in V.mungo. The of germination in treatment with0.906 solution was found to be 54 in H.annus,20 in L.esculentum,58 in T.aestivum,30 in O. sativa and 16 in V.mungo whereas the of germination in treatment with 1.188 solution was found out to be68 in H.annus,20 in L.esculentum,66 in T.aestivum,88 in O.sativa and 24 in V.mungo. Statistical analysis shows significant inhibition of germination in all cases with a good correlation between the concentration and germination percentage except in cases of O.sativa and V. mungo. The extract may be stimulatory at higher concentration. Field treatment to eliminate or prevent the formation of phytotoxins after the crop harvest may be suggested to minimize the autointoxication exhibited by some crop residues like the above.